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klenow fragment  (New England Biolabs)


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    Structured Review

    New England Biolabs klenow fragment
    Klenow Fragment, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 7141 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/klenow+fragment/Klenow+Fragment/pm42913629-186-3-10
    Average 99 stars, based on 7141 article reviews
    klenow fragment - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Infectious Pancreatic Necrosis in Atlantic Cod (Gadus morhua): Complete Viral Genome Characterization From the First Norwegian Field Case Suggests Cross-Species Transmission.
    Article Snippet: The RNA was further purified using the RNA Clean and Concentrator kit (Zymo Research). .. Reverse transcription and subsequent random amplification were performed with the SuperscriptTM IV First- Strand Synthesis System (Invitrogen, now ThermoFisher) and Klenow fragment (NEB). .. The DNAclearTM Purification Kit (ThermoFisher) was used to purify cDNA.

    Amplification:

    Article Title: Infectious Pancreatic Necrosis in Atlantic Cod (Gadus morhua): Complete Viral Genome Characterization From the First Norwegian Field Case Suggests Cross-Species Transmission.
    Article Snippet: The RNA was further purified using the RNA Clean and Concentrator kit (Zymo Research). .. Reverse transcription and subsequent random amplification were performed with the SuperscriptTM IV First- Strand Synthesis System (Invitrogen, now ThermoFisher) and Klenow fragment (NEB). .. The DNAclearTM Purification Kit (ThermoFisher) was used to purify cDNA.

    Produced:

    Article Title: Single Staphylococcus aureus SauUSI restriction enzyme motors fragment methylated DNA.
    Article Snippet: .. Biotinylated handles were produced by incubating Lambda DNA (dam−;dcm−, Thermo Fisher Scientific) with Klenow fragment (3ʹ → 5ʹ exo, NEB), 50 μM dATP/dTTP/dGTP and 40 μM biotin-14-dCTP in NEB buffer 2 at 37 °C for 30 min. After heat inactivation at 75 °C for 15 min, the DNA was digested with BsaI-HFv2 (NEB) at 37 °C for 3 h and the 11.4-kb fragment was purified following agarose gel electrophoresis using QIAEX II beads (Qiagen). ..

    Lambda DNA Preparation:

    Article Title: Single Staphylococcus aureus SauUSI restriction enzyme motors fragment methylated DNA.
    Article Snippet: .. Biotinylated handles were produced by incubating Lambda DNA (dam−;dcm−, Thermo Fisher Scientific) with Klenow fragment (3ʹ → 5ʹ exo, NEB), 50 μM dATP/dTTP/dGTP and 40 μM biotin-14-dCTP in NEB buffer 2 at 37 °C for 30 min. After heat inactivation at 75 °C for 15 min, the DNA was digested with BsaI-HFv2 (NEB) at 37 °C for 3 h and the 11.4-kb fragment was purified following agarose gel electrophoresis using QIAEX II beads (Qiagen). ..

    Inactivation:

    Article Title: Single Staphylococcus aureus SauUSI restriction enzyme motors fragment methylated DNA.
    Article Snippet: .. Biotinylated handles were produced by incubating Lambda DNA (dam−;dcm−, Thermo Fisher Scientific) with Klenow fragment (3ʹ → 5ʹ exo, NEB), 50 μM dATP/dTTP/dGTP and 40 μM biotin-14-dCTP in NEB buffer 2 at 37 °C for 30 min. After heat inactivation at 75 °C for 15 min, the DNA was digested with BsaI-HFv2 (NEB) at 37 °C for 3 h and the 11.4-kb fragment was purified following agarose gel electrophoresis using QIAEX II beads (Qiagen). ..

    Purification:

    Article Title: Single Staphylococcus aureus SauUSI restriction enzyme motors fragment methylated DNA.
    Article Snippet: .. Biotinylated handles were produced by incubating Lambda DNA (dam−;dcm−, Thermo Fisher Scientific) with Klenow fragment (3ʹ → 5ʹ exo, NEB), 50 μM dATP/dTTP/dGTP and 40 μM biotin-14-dCTP in NEB buffer 2 at 37 °C for 30 min. After heat inactivation at 75 °C for 15 min, the DNA was digested with BsaI-HFv2 (NEB) at 37 °C for 3 h and the 11.4-kb fragment was purified following agarose gel electrophoresis using QIAEX II beads (Qiagen). ..

    Agarose Gel Electrophoresis:

    Article Title: Single Staphylococcus aureus SauUSI restriction enzyme motors fragment methylated DNA.
    Article Snippet: .. Biotinylated handles were produced by incubating Lambda DNA (dam−;dcm−, Thermo Fisher Scientific) with Klenow fragment (3ʹ → 5ʹ exo, NEB), 50 μM dATP/dTTP/dGTP and 40 μM biotin-14-dCTP in NEB buffer 2 at 37 °C for 30 min. After heat inactivation at 75 °C for 15 min, the DNA was digested with BsaI-HFv2 (NEB) at 37 °C for 3 h and the 11.4-kb fragment was purified following agarose gel electrophoresis using QIAEX II beads (Qiagen). ..

    other:

    Article Title: Protocol for Cas9 nickase-mediated induction and two-dimensional gel detection of i-loops at human telomeric and alpha satellite repeats.
    Article Snippet: E. coli One Shot Stbl3 chemically competent cells Invitrogen Cat# C737303 Lentiviral particles: Lenti-iCas9-neo (parent backbone) Addgene Cat# 85400; RRID: Addgene_85400 Lentiviral particles: LentiGuide-Puro (sgRNA backbone) Addgene Cat# 52963; RRID: Addgene_52963 Chemicals, peptides and recombinant proteins Agarose (standard grade) Euroclone Cat# EMR010500 Agarose Low EEO (for 2D-gels and alkaline gels) US Biological Cat# A1015 Agarose, low melting point (for plug casting) Lonza Cat# 50104 Ampicillin Sigma-Aldrich Cat# A9518 Boric acid VWR Cat# 20185 Bromocresol green Sigma-Aldrich Cat # 114359 Bromophenol blue Sigma-Aldrich Cat# B0126 BSA (molecular biology grade) NEB Cat# B9000 BsmBI-v2 NEB Cat# R0739S CaCl2 Sigma-Aldrich Cat# C1016 Chloroform (molecular biology grade) Sigma-Aldrich Cat# C2432 CutSmart Buffer (10x) NEB Cat# B6004S Dimethyl sulfoxide (DMSO), sterile Sigma-Aldrich Cat# D2438 DNase I (RNase-free, lyophilized) Worthington Cat# LS006333 dNTP Set (100 mM solutions) Invitrogen Cat# 10297018 Doxycycline hydrochloride Sigma-Aldrich Cat# D9891; CAS: 24390-14-5 DpnI restriction enzyme NEB Cat# R0176L DTT (dithiothreitol), 1 M Sigma-Aldrich Cat# D9779 EDTA Sigma-Aldrich Cat# E9884 EGTA Sigma-Aldrich Cat# E3889 Ethanol, absolute (molecular biology grade) Sigma-Aldrich CAS: 64-17-5 Ethidium Bromide solution (10 mg/mL) Sigma-Aldrich Cat# E1510 Ficoll 400 Sigma-Aldrich Cat# F4375 Geneticin G418 sulfate (powder) Life Technologies Cat# 11811031 Glycerol (molecular biology grade) Sigma-Aldrich Cat# G5516 HCl, 37% (12 N) Sigma-Aldrich Cat# 320331 HinfI NEB Cat# R0155L Isopropanol (molecular biology grade) Sigma-Aldrich CAS: 67-63-0 KCl Sigma-Aldrich Cat# P9333 Klenow Fragment (3’→5′ exo-) NEB Cat# M0212S L-Glutamine, 200 mM Euroclone Cat# LOBE1760F LB Broth, Miller BD Diagnostic Systems Cat# 244620 Magnesium acetate Sigma-Aldrich Cat# M5661 MgCl2 Sigma-Aldrich Cat# M8266 MspI NEB Cat# R0106L N-Lauroylsarcosine sodium salt Sigma-Aldrich Cat# L9150 NaOH Sigma-Aldrich Cat# 1.09137 Phenol:chloroform:isoamyl alcohol (25:24:1) Sigma-Aldrich Cat# P2069 PMSF (phenylmethylsulfonyl fluoride) Sigma-Aldrich Cat# 78830 Polybrene (Hexadimethrine bromide) Sigma-Aldrich Cat# H9268 Polyethylenimine Max 40000 (PEI Max) Histo-Line Laboratories Cat# 24765-1 Potassium phosphate monobasic Sigma-Aldrich Cat# P0662 Protease Inhibitor Cocktail (cOmplete, EDTA-free) Roche Cat# 11836170001 Proteinase K (20 mg/mL stock solution) Roche Cat# 3115887001 Puromycin dihydrochloride Adipogen Cat# AG-CN2-0078-M100 Q5 High-Fidelity DNA Polymerase NEB Cat# M0491S Quick Ligation Kit NEB Cat# M2200S RNase A (10 mg/mL, DNase-free) Sigma-Aldrich Cat# R5000 RsaI NEB Cat# R0167L (Continued on next page) 6 STAR Protocols 7, 104888, December 18, 2026

    Ligation:

    Article Title: C. elegans CEH-43/DLX drives convergent astrocyte differentiation by sequential control of axon guidance and synaptic gene expression programs.
    Article Snippet: DamID adaptors were removed with AlwI (NEB) followed by one round of bead purification. .. End repair was performed using an enzyme mix of T4 DNA polymerase, Klenow fragment, and T4 polynucleotide kinase (NEB), followed by 3′ adenylationwith Klenow fragment (3′ →5′ exo−) (NEB) and ligation of indexed sequencing adaptors. .. Libraries were bead-purified twice, amplified with NEBNext PCRmaster mix, purified again, checked for quality on a TapeStation, and pooled for Illumina sequencing.

    Sequencing:

    Article Title: C. elegans CEH-43/DLX drives convergent astrocyte differentiation by sequential control of axon guidance and synaptic gene expression programs.
    Article Snippet: DamID adaptors were removed with AlwI (NEB) followed by one round of bead purification. .. End repair was performed using an enzyme mix of T4 DNA polymerase, Klenow fragment, and T4 polynucleotide kinase (NEB), followed by 3′ adenylationwith Klenow fragment (3′ →5′ exo−) (NEB) and ligation of indexed sequencing adaptors. .. Libraries were bead-purified twice, amplified with NEBNext PCRmaster mix, purified again, checked for quality on a TapeStation, and pooled for Illumina sequencing.



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